Transformation of Streptococcus sanguis Challis with Streptococcus lactis plasmid DNA
نویسندگان
چکیده
منابع مشابه
Transformation of Streptococcus lactis Protoplasts by Plasmid DNA.
Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.
متن کاملHigh-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.
Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).
متن کاملRecombination-deficient Streptococcus sanguis.
A UV-sensitive derivative was obtained from Streptococcus sanguis Challis. The organism could be transformed with a number of small streptococcal plasmids at frequencies equal to, or 1 logarithm below, the transformation frequencies for the parent organism. However, transformation with chromosomal DNA was greatly impaired in the UV-sensitive derivative.
متن کاملTransformation of Streptococcus sanguis to intrinsic penicillin resistance.
A series of step-level penicillin-resistant derivatives of Streptococcus sanguis V288 (Challis) were obtained through successive genetic transformations. The DNA donor used was a laboratory-derived, penicillin-resistant multistep mutant of the recipient strain. Detection of the penicillin-binding proteins (PBPs) of wild-type and transformants revealed five major PBPs. While it was found that S....
متن کاملProphylactic Immunization against Streptococcus sanguis.
Streptococcus sanguis (the Streptococcus s.b.e. of Loewe), originally identified by White and his coworkers, has, with few exceptions, been isolated only from the blood stream of patients with subacute bacterial endocarditis (White, 1944; White and Niven, 1946; Niven and White, 1946; Loewe et al., 1946; Niven et al., 1946; Washburn et al., 1946). This causative organism has a type specificity a...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1984
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.48.2.342-346.1984